Workshop: Stain & Sustain – Mastering IMMUNOHISTOCHEMISTRY in Diagnostic Pathology

Target Audience: Pathology Postgraduates and Consultants

Venue: Department of Pathology, JSS Hospital, Mysuru

No of participants: 20

Venue: Department of Pathology, JSS Hospital, Mysuru

Target Audience: Pathology Postgraduates and Consultants

No of participants: 20

Time	Session	Format
08:30 – 09:00 AM	Registration	Registration
09:00 – 09:15 AM	Registration & Pre-Test Participant registration 10 MCQ pre-assessment to assess baseline knowledge in IHC	Assessment
09:15 – 09:30 AM	Introduction Objectives of the workshop Role of Immunohistochemistry in modern diagnostic pathology	Opening Session
09:30 – 10:15 AM	Session 1: Understanding Immunohistochemistry – Multiutility Tool or Double-Edged Sword? Principle of antigen-antibody reaction Evolution of IHC in diagnostic pathology Indications and limitations Pre-analytical, analytical and post-analytical variables Common pitfalls in interpretation	Lecture
10:15 – 11:00 AM	Session 2: IHC Technique – From Slide to Signal Tissue fixation and processing Antigen retrieval methods Manual IHC staining procedure Detection systems Chromogens and counterstaining Quality control measures	Lecture
11:00 – 11:15 AM	Tea Break
11:15 AM – 12:15 PM	Session 3: Organ-Based IHC Approach Immunohistochemistry of Lymph Node Lymphoma panels Reactive vs Neoplastic differentiation Immunohistochemistry of Breast ER, PR, HER2 interpretation Predictive and prognostic markers	Lecture
12:15 – 01:00 PM	Live Demonstration (Laboratory Session) Demonstration of Manual IHC Staining Procedure Overview of Automated IHC System Demonstration on Leica Bond Max Troubleshooting common technical issues Instrument maintenance basics	Live Demonstration
01:00 – 02:00 PM	Lunch Break
02:00 – 03:00 PM	Session 4: Carcinoma of Unknown Primary – What is the Most Specific Antibody? Stepwise panel selection Cytokeratin profiling (CK7/CK20 patterns) Organ-specific markers Role of morphology in guiding IHC Algorithm-based diagnostic approach	Lecture
03:00 – 03:15 PM	Session 5: Quality Assurance & Reporting in IHC Internal quality control External quality assurance programs Standard reporting formats Interpretation scoring systems Documentation and accreditation requirements	Lecture
03:15 – 03:45 PM	Hands-On Interpretation Exercise Participants divided into small groups Digital/printed IHC case slides provided Panel selection exercise Group discussion and presentation Faculty feedback	Hands-On Session
03:45 – 05:00 PM	Post-Test & Discussion	Assessment & Closing