Workshop:
PEAK-A-BOO – DETECTING HEMOGLOBINOPATHIES BY HPLC

Venue: Department of Biochemistry, JSS Hospital, Mysuru

Target Audience: Pathology postgraduates and consultants

No of participants: 20

Venue: Department of Biochemistry, JSS Hospital, Mysuru

Target Audience: Pathology postgraduates and consultants

No of participants: 20

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Time Session Speakers Format
08:30 – 09:00 AM Registration Registration
09:00 – 09:15 AM Pre-Test
  • 10 MCQ pre-assessment to assess baseline knowledge
 Assessment
09:15 – 09:30 AM Introduction
  • Objectives of the workshop
  • Overview of hemoglobin disorders burden in India
 Dr. Jayashree D. Kulkarni Opening Session
09:30 – 10:15 AM Session 1: Basics of Hemoglobinopathies
  • Structure of hemoglobin
  • Genetics of globin chain synthesis
  • Classification – Thalassemias, HbS, HbD, HbE, HbC
  • Diagnostic approach to Hemoglobinopathies
 Dr. Gayathri J. Lecture
10:15 – 11:00 AM Session 2: Principles of HPLC in Hemoglobin Analysis
  • Principle of cation-exchange HPLC
  • Instrument components
  • Retention time concept
  • Calibration & quality control
  • Comparison with other methods
 Dr. Jayashree D Kulkarni Lecture
11:00 – 11:15 AM Tea Break
11:15 AM – 12:15 PM Session 3: Understanding HPLC Chromatograms
  • Normal adult & neonatal chromatograms
  • Interpretation of peaks – HbA0, HbA2, HbF, Variant windows
  • Diagnostic patterns of various hemoglobinopathies
  • Pitfalls & borderline cases
  • Hemoglobin variants detected during HbA1c testing
 Dr. Jayashree D. Kulkarni Lecture
12:15 – 01:00 PM Case Scenarios Dr. Swetha N K & Dr. Kusuma K S Discussion
01:00 – 02:00 PM Lunch Break
02:00 – 03:00 PM Session 4: Case-Based Interactive Discussion
  • 6–8 anonymized real cases
  • Stepwise interpretation approach
  • Correlation with CBC indices
  • Molecular testing recommendations
  • Importance of antenatal screening
 Dr. Gayathri J. Interactive Discussion
03:00 – 03:15 PM Session 5: Quality Assurance & Reporting
  • Internal quality control
  • External quality assurance programs
  • Reporting formats & critical values
  • Counselling implications
 Dr. Jayashree D. Kulkarni Lecture
03:15 – 03:45 PM Hands-On Interpretation Exercise
  • Small group interpretation
  • Printed chromatograms
  • Faculty feedback
 Dr. Jayashree D. Kulkarni Hands-on
03:45 – 05:00 PM Post-Test & Discussion Assessment & Closing

Resource Faculty

Dr. Jayashree D. Kulkarni

MBBS., MD(Pathology), MBA(HCS)

HOD Hematopathology and Director–Lab services and Quality

Sri Shankara Cancer Hospital and Research Centre

Bengaluru

Mobile – 9972461925.

Email – jayashreedk@gmail.com

Dr. Gayathri J

MBBS., MD(Pathology), PDCC(Advanced Hematology)

Consultant hematopathology

Sri Shankara Cancer Hospital and Research Centre

Bengaluru

Mobile – 9945441385.

Email – drgayathri39@gmail.com

Dr. Swetha N. K

MBBS, MD(Biochemistry), PhD.,
Department of Biochemistry,

JSS Medical College, JSS AHER,
Mysuru
Mobile – 9886403651
Email – swethank@jssuni.edu.in

Dr. Kusuma K S

Associate Professor,

Department of Biochemistry,

JSS Medical College, JSS AHER,

Mysuru
Mobile – 9902558907
Email – kusumaks@jssuni.edu.in

Dr. Deepika Gurumurthy

Senior Resident,

Department of Pathology,

JSS Medical College, JSS AHER,
Mysuru
Mobile – 9739320067
Email – deepikagurumurthy1410@gmail.com

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